- Patent Title: Transformant of Schizosaccharomyces pombe mutant and cloning vector
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Application No.: US14627459Application Date: 2015-02-20
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Publication No.: US09765347B2Publication Date: 2017-09-19
- Inventor: Mayumi Kobayashi , Hideki Tohda , Shuichiro Kimura , Susumu Zen-In , Futoshi Hara
- Applicant: Asahi Glass Company, Limited
- Applicant Address: JP Tokyo
- Assignee: Asahi Glass Company, Limited
- Current Assignee: Asahi Glass Company, Limited
- Current Assignee Address: JP Tokyo
- Agency: Oblon, McClelland, Maier & Neustadt, L.L.P.
- Priority: JP2012-181865 20120820
- Main IPC: C12N15/81
- IPC: C12N15/81 ; C12N9/42

Abstract:
Provided is a transformant of S. pombe mutant which can produce and collect β-glucosidase without requiring complicated separation steps, and a vector which is useful for transforming a yeast of the genus Schizosaccharomyces. The transformant of a S. pombe mutant of the present invention is a transformant of a Schizosaccharomyces pombe mutant which exhibits an increased Gsf activity and decreased or no pyruvyl transferase Pvg1 enzymatic activity, and is comprised of a structural gene sequence encoding a β-glucosidase derived from a filamentous fungus, and a promoter sequence and a terminator sequence for expressing the structural gene in a chromosome or as an extrachromosomal gene. Further, the present invention relates to a cloning vector which is characterized by comprising a hsp9 gene promoter or an ihc1 gene promoter of a yeast of the genus Schizosaccharomyces, and is useful for transforming a yeast of the genus Schizosaccharomyces, an expression vector, a transformant, etc.
Public/Granted literature
- US20150240250A1 TRANSFORMANT OF SCHIZOSACCHAROMYCES POMBE MUTANT AND CLONING VECTOR Public/Granted day:2015-08-27
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