An optical film includes a substrate, an anti-glare layer, a first anti-reflective layer and a second anti-reflective layer. The anti-glare layer is disposed on the substrate and has a microstructure disposed on a side of the anti-glare layer away from the substrate. The first anti-reflective layer is disposed on the microstructures of the anti-glare layer, and the anti-glare layer is located between the substrate and the first anti-reflective layer. The second anti-reflective layer is disposed on the first anti-reflective layer, and the first anti-reflective layer is located between the anti-glare layer and the second anti-reflective layer. A refractive index of the first anti-reflective layer is different from a refractive index of the anti-glare layer.