A microlens array substrate includes: a light transmitting substrate in which a first lens surface formed of a concave surface is formed on a substrate surface on one side; a first lens layer which covers the substrate surface on one side and has a refractive index which is different from that of the light transmitting substrate; a light transmitting layer which covers the first lens layer on the opposite side to the light transmitting substrate; and a second lens layer which covers the light transmitting layer on the opposite side to the light transmitting substrate and in which a second lens surface formed of a convex surface is formed on the opposite side to the light transmitting substrate, in which the light transmitting layer has smaller refractive index and coefficient of thermal expansion than those of the first lens layer and the second lens layer.