Embodiments of the present invention disclose a double-faced display panel and a manufacturing method thereof, the double-faced display panel includes: an array substrate, including a first transparent substrate, a first trans-reflective layer formed on the first transparent substrate and a first electrode layer formed on the first trans-reflective layer; an opposed substrate, disposed to face the array substrate, and including a second transparent substrate, a second trans-reflective layer formed on a side of the second transparent substrate facing the first transparent substrate and a second electrode layer formed on a side of the second transparent substrate facing the first transparent substrate; a display structure, disposed between the array substrate and the opposed substrate and including a plurality groups of display units arranged in matrix, wherein each of the first and second trans-reflective layers includes a plurality of reflection regions and a plurality of transmission regions disposed alternately.