The present disclosure relates to a fabricating method of alignment film. The fabricating method of alignment film comprises: forming a blocking layer (2) on a substrate (1); and forming an alignment layer (3) on the blocking layer (2). According to the present disclosure, by way of a layered coating technology, a complete separation of the blocking layer and the alignment layer is achieved. Moreover, since the blocking layer is coated in advance, the alignment layer has a decreased angle with respect to the substrate, which facilitates a uniform distribution of the alignment layer such that the formation of Mura on the panel is effectively avoided. In addition, the blocking layer and the alignment layer are formed in different steps such that the thickness of the blocking layer and the alignment layer is accurately controlled. Therefore, an accurate distribution of capacitance on the blocking layer and the alignment layer is achieved and the residual charge on the alignment layer is effectively reduced.