An interlock data collection and calibration system has a device computer, a gas delivery system, and a data port. The device computer has a computer processor and a computer memory. The gas delivery system delivers a gas sample to an ignition interlock device. The data port is operably connected with the device computer for enabling sample data from the ignition interlock device to be transmitted to the device computer. A calibration program operably installed on the computer memory receives the sample data, calibrates the ignition interlock device, and generates confirmation data, which is stored in a local database.