In an alcohol concentration estimation apparatus for an engine, a first crank angular speed (ω1) within a first predetermined interval overlapping with the compression top dead center is calculated, and a first variation amount (Δω1) is calculated by subtracting ω1 from an average engine speed. A second crank angular speed (ω2) within a second predetermined interval overlapping with the combustion bottom dead center is calculated, and a second variation amount (Δω2) is calculated by subtracting ω1 from ω2. In a relationship between air fuel ratio A/F and an indicated mean effective pressure (IMEP)/charging efficiency (ηc) of the engine when the engine is operated with a predetermined fuel injection map indicating a relationship between A/F and IMEP/ηc for each desired alcohol concentration, IMEP/ηc is substituted by Δω2/Δω1 ratio to estimate an alcohol concentration in fuel. The alcohol concentration estimation apparatus eliminates requirement of sensors for detecting an intake air mass.