The present invention discloses a color filter substrate and a manufacturing method thereof and a touch screen. The color filter substrate comprises a substrate and a black matrix arranged thereon, the black matrix defines a plurality of pixel units, in each of which a color filter is arranged; the color filter substrate further comprises light sensing units as well as touch scan lines and touch sensing lines insulated from each other, the light sensing units are positioned in the pixel units; the light sensing units, the touch scan lines and the touch sensing lines are arranged in areas corresponding to the black matrix; and each light sensing unit is connected with one touch scan line and one touch sensing line and is positioned on one side of the black matrix that can receive external light.