Invention Grant
- Patent Title: Inducible gene expression composition for using eukaryotic pol-2 promoter-driven transcription in prokaryotes and the applications thereof
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Application No.: US14502608Application Date: 2014-09-30
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Publication No.: US09783811B2Publication Date: 2017-10-10
- Inventor: Shi-Lung Lin , Donald C. Chang
- Applicant: MELLO BIOTECHNOLOGY, INC.
- Applicant Address: US CA Santa Fe Springs
- Assignee: MELLO BIOTECHNOLOGY, INC.
- Current Assignee: MELLO BIOTECHNOLOGY, INC.
- Current Assignee Address: US CA Santa Fe Springs
- Agency: Birch, Stewart, Kolasch & Birch, LLP
- Main IPC: C12N15/74
- IPC: C12N15/74 ; C12P21/06 ; C12N15/63 ; C12N1/38 ; C12N15/70 ; A01K67/00

Abstract:
Eukaryotic protein-coding messenger RNAs and non-coding microRNAs are naturally transcribed by type II RNA polymerases (pol-2) but not prokaryotic RNA polymerases. As a result, current eukaryotic RNA and protein production is performed either using eukaryotic pol-2 promoters in hybridomas or mammalian cells or using prokaryotic promoters in bacterial cells. However, because prokaryotic RNA transcription tends to be error-prone, frequent mutation is a big problem. Also, growing hybridomas or mammalian cells is relatively laborious and costly. To overcome these problems, the present invention provides a novel inducible composition and method for producing eukaryotic RNAs and/or their related peptides/proteins directly using eukaryotic pol-2 promoter-driven gene expression in fast growing bacteria, without the need of changing to prokaryotic promoters or growing hybridomas/mammalian cells. The RNAs and peptides/proteins so obtained can be used to develop drugs, cure diseases, treat tumors/cancers, produce pluripotent stem (iPS) cells, enhance wound healing, and make foods.
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