Various aspects of a method and a system for segmentation of vascular structure in a volumetric image dataset are disclosed herein. The method comprises initial segmentation of an input volumetric image dataset to obtain a main vessel structure and a plurality of broken segments. A weighted path is computed between the main vessel structure and a broken segment of the plurality of broken segments. The computation of the weighted path is based on at least one or more parameters associated with a first voxel of the broken segment and a second voxel of the main vessel structure. A valid, weighted path is determined between the main vessel structure and a broken segment of the plurality of broken segments, based on the computed weighted path and one or more pre-specified conditions. Based on the determined valid, weighted path, an output volumetric image dataset is generated by performance of a final segmentation on a gradient field.