The subject matter described herein relates to method, systems, and computer readable media for detecting physical link intrusions. A method for detecting physical link intrusions includes monitoring a link delay signature associated with a physical link based on one-way link delay measurements obtained using at least one network tap. The method also includes determining whether a change in the link delay signature has met or exceeded a threshold value. The method further includes in response to determining that the change in the link delay signature has met or exceeded the threshold value, determining that a physical link intrusion has occurred.