A method for determining a point spread function (PSF) of a camera, comprising: displaying on a display at least two images comprising visual features, elements having a different optical intensity, and a test pattern; acquiring the images using the camera; determining a coordinate transformation using the image comprising the visual features and its corresponding acquired image, the coordinate transformation for aligning together a coordinate system of the camera and a coordinate system of the display; aligning the acquired image comprising the elements having a different optical intensity using the determined coordinate system and determining a difference of intensity between the displayed image comprising the elements having a different optical intensity and the corresponding acquired image; aligning the acquired image comprising the test pattern and correcting an intensity of the acquired image comprising the test pattern, thereby obtaining a corrected image; and estimating a PSF using the corrected image.