Invention Grant
- Patent Title: Spatial molecular barcoding of in situ nucleic acids
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Application No.: US14493127Application Date: 2014-09-22
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Publication No.: US09834814B2Publication Date: 2017-12-05
- Inventor: Brian Jon Peter , Robert A. Ach , Alicia Scheffer-Wong , Carolina Caffaro
- Applicant: Agilent Technologies, Inc.
- Applicant Address: US CA Santa Clara
- Assignee: Agilent Technologies, Inc.
- Current Assignee: Agilent Technologies, Inc.
- Current Assignee Address: US CA Santa Clara
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; G01N33/53 ; C07H21/00

Abstract:
This disclosure provides, among other things, a method for analyzing a planar cellular sample. In some embodiments, the method comprises: (a) indirectly or directly attaching nucleic acid tags to binding sites in a planar cellular sample; (b) contacting the planar cellular sample with a solid support comprising an array of spatially addressed features that comprise oligonucleotides, wherein each oligonucleotide comprises a molecular barcode that identifies the feature in which the oligonucleotides is present; (c) hybridizing the nucleic acid tags, or a copy of the same, with the oligonucleotides to produce duplexes; and (d) extending the oligonucleotides in the duplexes to produce extension products that each comprises (i) a molecular barcode and (ii) a copy of a nucleic acid tag. Other embodiments, e.g., kits and the like, are also described.
Public/Granted literature
- US20150148239A1 SPATIAL MOLECULAR BARCODING OF IN SITU NUCLEIC ACIDS Public/Granted day:2015-05-28
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