A urine specimen analysis device includes a specimen drawing portion, a sample preparing portion, a measurement portion, and an information processing portion. The specimen drawing portion draws a first aliquot and a second aliquot from a urine specimen. The sample preparing portion prepares a first measurement sample by mixing the first aliquot and a first staining dye that stains red blood cells, and a second measurement sample by mixing the second aliquot and a second staining dye that stains nucleic acids. The measurement portion measures fluorescence emitted from the first measurement sample prepared by the sample preparing portion, and fluorescence emitted from the second measurement sample prepared by the sample preparing portion. The information processing portion detects at least red blood cells contained in the first measurement sample based on the fluorescence of the first measurement sample measured by the measurement portion, and at least white blood cells contained in the second measurement sample based on the fluorescence of the second measurement sample measured by the measurement portion.