The redox flow secondary battery includes an electrolytic cell including a positive electrode cell, a negative electrode cell, and a separation membrane that separates the positive electrode cell and the negative electrode cell. Moreover, the above described redox flow secondary battery is configured as follows. That is, the separation membrane has a microporous membrane and an ion-exchange resin layer contacting the microporous membrane, and the air resistance of the separation membrane per thickness of 200 μm is 10000 sec/100 cc or more. Furthermore, the microporous membrane includes a polyolefin resin or a vinylidene fluoride resin and an inorganic filler. Further, the smoothness of at least a surface of the microporous membrane contacting the ion-exchange resin layer is 16000 seconds or less.