A serial peripheral interface is configurable to operate in a I2S transmission mode. The interface has a transmission unit connected with external pins for data, bit clock, and left/right clock signal, a first-in-first-out (FIFO) buffer with a plurality of memory lines, and a control unit operable to read data portions from two memory lines, to assemble them into a transmission word, and to forward the assembled transmission word to the transmission unit, wherein the transmission unit is configured to serially transmit the assembled transmission word through the external data pin.