A speaker device with an acoustic tuning assembly can adjust sound in variety. The acoustic tuning assembly includes a main body and a housing receiving the main body. The main body receives an acoustic unit, and includes a first sound chamber and an upper peripheral wall. The housing defines a plurality of through holes. The upper peripheral wall defines a plurality of match holes. The housing can be rotated relatively to the main body to make the through holes of the housing overlapped partially or completely with the match holes to form a plurality of overlap holes, or make the through holes of the housing non-overlapping with the match holes. Therefore, sound from the acoustic unit can be transmitted through the first sound chamber and selectively through the overlap holes.