Methods of and devices for manufacturing a multi-layered microfluidic filter are disclosed. In one embodiment, method of manufacturing a multi-layered filter comprises providing a first molding plate that includes a plurality of apertures and is coupled to a flow stream source, applying from the flow stream source a first flow stream to pass through the plurality of apertures of the first molding plate, forming a first membrane layer comprising a first set of pores using the first molding plate and the first flow stream, controlling the first flow stream to generate a second flow stream from the first set of pores of the first membrane layer, and forming a second membrane layer comprising a second set of pores using the second flow stream and the first membrane layer.