A three-dimensional printing device including a processing unit and a mixing unit is provided. The processing unit formats a sliced file into a printing head signal file, wherein the sliced file and the printing head signal file correspond to a three-dimensional structure, the sliced file includes layer data corresponding to a plurality of layers of the three-dimensional structure, and the print head signal file includes layer printing data corresponding to the layers of the three-dimensional structure. The mixing unit combines part of the layer data in the sliced file and part of the layer printing data in the printing head signal file to derive a hybrid file, and stores the hybrid file in a storing unit of the electronic device, wherein the layer data and the layer printing data in the hybrid file correspond to different layers of the three-dimensional structure.