Invention Grant
- Patent Title: High fidelity restriction endonucleases
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Application No.: US15133093Application Date: 2016-04-19
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Publication No.: US09856464B2Publication Date: 2018-01-02
- Inventor: Zhenyu Zhu , Aine Quimby , Shengxi Guan , Dapeng Sun , Yishu Huang , Xuhui Lai , Siu-hong Chan , Xianghui Li , Shuang-Yong Xu , Chunhua Zhang
- Applicant: New England Biolabs, Inc.
- Agency: New England Bio Labs, Inc.
- Agent Harriet M. Strimpel
- Main IPC: C12N9/22
- IPC: C12N9/22 ; C12Q1/34 ; C12N9/16 ; C12Q1/68 ; C12Q1/44
Abstract:
Methods and compositions are provided for engineering mutant enzymes with reduced star activity where the mutant enzymes have a fidelity index (FI) in a specified buffer that is greater than the FI of the non-mutated enzyme in the same buffer.
Public/Granted literature
- US20160289660A1 High Fidelity Restriction Endonucleases Public/Granted day:2016-10-06
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