Embodiments of the present disclosure relate to the display technical field, and disclose a display substrate and a liquid crystal display device, which will improve the pressure resistant performance of the liquid crystal display device, while not substantially reducing the LC margin of the liquid crystal display device. The display substrate comprises a base substrate and spacers disposed on the base substrate; the spacers at least comprise main spacers, auxiliary spacers and first sub-spacers; the plurality of main spacers encircle to form a repeating unit, in which a plurality of the auxiliary spacers are arranged in an array, and at least one first sub-spacer is distributed in a center region of the repeating unit; wherein the vertical distances from the top of the main spacers, the top of the first sub-spacers, the top of the auxiliary spacers to the base substrate decrease sequentially.