An environmentally friendly aerosol sealing composition for tire repair can simultaneously repair a punctured flat tire and inflate the flat tire to a pressure suitable for driving. The tire sealant composition includes a sealant concentrate and an aerosol propellant. The sealant concentrate is water-based and includes a glycerin antifreeze agent and a natural rubber latex that suitably has a mechanical stability of at least about 1050 seconds, and is substantially free of coagulants.