Invention Grant
- Patent Title: Method for determining the repair activity of non-homologous end joining
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Application No.: US15310491Application Date: 2015-08-18
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Publication No.: US09863933B2Publication Date: 2018-01-09
- Inventor: Fenju Liu , Jie Du , Jiahua Yu , Haowen Zhang , Zengfu Shang , Yushuo Zhang
- Applicant: Soochow University
- Applicant Address: CN Suzhou
- Assignee: SOOCHOW UNIVERSITY
- Current Assignee: SOOCHOW UNIVERSITY
- Current Assignee Address: CN Suzhou
- Agency: SZDC Law P.C.
- Priority: CN201510478296 20150807
- International Application: PCT/CN2015/087321 WO 20150818
- International Announcement: WO2017/024602 WO 20170216
- Main IPC: C12N5/00
- IPC: C12N5/00 ; G01N33/50 ; C12Q1/44 ; C12N15/90 ; C12N15/11
Abstract:
The invention discloses a method for determining the repair activity of NHEJ. In this method, HPRT gene is mutated by using a site-directed gene mutation technology, and plasmid transfection, and 6-TG treatment are performed. By means of the method of the invention, the NHEJ repair activity level of cells can be observed by measuring the cell viability. The method can be used for screening the effects of different drugs and different genes on the NHEJ repair activity.
Public/Granted literature
- US20170254799A1 Method for Determining the Repair Activity of Non-Homologous End Joining Public/Granted day:2017-09-07
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