A position aligning apparatus of a vehicle installed at a bottom surface of a vehicle inspection line to respectively correspond to both sides of a front wheel and both sides of a rear wheel of the vehicle so as to target-position the vehicle at an inspection position includes: a front aligning unit regulating a front movement of the vehicle having entered into the vehicle inspection line and aligning a front right/left position of the vehicle while pressing a front wheel of the vehicle in a right/left direction according to operation of a first actuator; a rear aligning unit aligning a rear right/left position of the vehicle while pressing a rear wheel of the vehicle in the right/left direction according to operation of a second actuator; and a controller controlling the operation of the first and second actuators.