Disclosed are systems and methods for protecting transmission of audio data from microphone to application process. An exemplary method includes receiving a request from a software process to obtain an audio stream from an audio endpoint device; allocating a data buffer for the software process; processing and encrypting audio data received from the audio endpoint device by audio processing objects; storing the encrypted audio data in the allocated data buffer; installing an interceptor of a API function call for the software process; and decrypting the encrypted audio data from the allocated data buffer by the software process using the interceptor of the API function call.