Invention Grant
- Patent Title: Protein discovery using intracellular ribosome display
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Application No.: US12671446Application Date: 2008-07-31
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Publication No.: US09879252B2Publication Date: 2018-01-30
- Inventor: Matthew P. Delisa , Lydia Contreras-Martinez
- Applicant: Matthew P. Delisa , Lydia Contreras-Martinez
- Applicant Address: US NY Ithaca
- Assignee: Cornell Research Foundation, Inc.
- Current Assignee: Cornell Research Foundation, Inc.
- Current Assignee Address: US NY Ithaca
- Agency: LeClairRyan, a Professional Corporation
- International Application: PCT/US2008/071747 WO 20080731
- International Announcement: WO2009/018438 WO 20090205
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12N15/10

Abstract:
The present invention relates to a method of identifying a protein that binds to a target molecule and has intracellular functionality. This method includes providing a construct comprising a deoxyribonucleic acid molecule encoding the protein which binds to the target molecule, with the deoxyribonucleic acid molecule being coupled to a stall sequence. A host cell is transformed with the construct and then cultured under conditions effective to form, within the host cell, a complex of the protein whose translation has been stalled, the mRNA encoding the protein, and ribosomes. The protein in the complex is in a properly folded, active form and the complex is recovered from the cell.
Public/Granted literature
- US20110008774A1 PROTEIN DISCOVERY USING INTRACELLULAR RIBOSOME DISPLAY Public/Granted day:2011-01-13
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