The invention provides a high-performance liquid chromatography system, said system is controlled in temperature by running a fluid in sleeves that surround the different parts of the system. All parts in contact with the fluid are made in fluoropolymer, carbon-filled fluoropolymer, or carbon-fiber fluoropolymer. The system comprises at least one reagent reservoir; at least one mixing chamber, wherein the contents of the at least one reagent reservoir are combined; at least one pump that transfers the contents of the at least one reservoir to the mixing chamber; and at least one modular elution column, wherein the at least one modular elution column contains a temperature control means; a sample injection system connected to an injection loop or 3-way valve to inject the sample solutions in the modular elution columns, at least one manifold or X-Y moving stage to distribute the eluted volumes in different receptacles; at least one return line to automatically reinject selected elution fractions at the top of the column; wherein all moving components of the said system are fluid actuated.