A self-adhesive floorboard with water-resistant glue comprises from a top to a bottom sequentially a main body layer, an isolation layer and the water-resistant glue, wherein each component proportion of component composition of the water-resistant glue based on a weight percentage is: 34-38% for a styrene-isoprene-styrene block copolymer, 46.4-50.4% for C5 hydrogenated petroleum resin, 13-17% for cycloalkyl rubber extender oil and 0.5-0.6% for a hindered phenol antioxidant. The isolation layer between the main body layer and the water-resistant glue is a PVC film of OPHR. The water-resistant glue of the PVC self-adhesive floorboard with the water-resistant glue is provided with a water-resistance function, and can prevent a plasticizer from drifting away from the PVC floorboard to affect stickiness of the water-resistant glue.