Invention Grant
- Patent Title: Carbohydrate binding modules with reduced binding to lignin
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Application No.: US14919199Application Date: 2015-10-21
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Publication No.: US09885028B2Publication Date: 2018-02-06
- Inventor: John J. Tomashek , Brian R. Scott , Daniel Kolczynski
- Applicant: Iogen Energy Corporation
- Applicant Address: CA Ottawa
- Assignee: Iogen Energy Corporation
- Current Assignee: Iogen Energy Corporation
- Current Assignee Address: CA Ottawa
- Agency: Fitzpatrick, Cella, Harper & Scinto
- Main IPC: C12N9/42
- IPC: C12N9/42 ; C12P19/14 ; C12P7/06 ; C12P19/02 ; C12N9/24

Abstract:
Provided is a modified Family 1 carbohydrate binding module (CBM) comprising amino acid substitutions at one or more of positions 10, 11, 12, 14, 17, 21, 24, 29, 31, 33, and 37, said position determined from alignment of a Family 1 CBM amino acid sequence with SEQ ID NO: 30, and exhibiting from about 50% to about 99.9% amino acid sequence identity to SEQ ID NO: 30. Also provided are modified glycosidase enzymes comprising the modified Family 1 CBM, genetic constructs and genetically modified microbes for expressing the modified Family 1 CBM or modified glycosidase enzyme. The modified Family 1 CBM confers reduced lignin binding and/or increased hydrolyzing activity in the presence of lignin to the modified glycosidase enzyme, which may be used in a process for hydrolyzing cellulose or hemicellulose in the presence of lignin.
Public/Granted literature
- US20160115464A1 CARBOHYDRATE BINDING MODULES WITH REDUCED BINDING TO LIGNIN Public/Granted day:2016-04-28
Information query
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