The present invention provides a chip for analysis of a target substance that is compact and allows analysis of a target substance with less time and effort. The chip for analysis of a target substance includes a first flexible substrate 1, a second flexible substrate 2, and a third substrate 3. A flow channel-forming non-bonded area 11 is formed on a bonding surface of the first flexible substrate 1 and the second flexible substrate 2 in a band-like manner and an extraction chamber-forming non-bonded area 5 having a wider band width than the flow channel-forming non-bonded area 11 is formed at a part of the flow channel-forming non-bonded area 11. The first flexible substrate 1 includes a through-hole 7 that is in contact with the flow channel-forming non-bonded area 11, a shutter-forming non-bonded area 12b is formed on a bonding surface of the second flexible substrate 2 and the third substrate 3 at a far side of the through-hole 7 relative to the extraction chamber-forming non-bonded area 5 such that the shutter-forming non-bonded area 12b and the flow channel-forming non-bonded area 11 intersect above and below, the first flexible substrate 1 and the second flexible substrate 2 include a pressure supply port 18b that comes through the substrates so as to be in contact with the shutter-forming non-bonded area 12b, and a magnetic particle 16 that binds to a target substance is placed above the extraction chamber-forming non-bonded area 5.