The invention provides an array substrate, comprising a base substrate, a first electrode layer comprising multiple first electrode strips arranged in a matrix, and a second electrode layer insulated from the first electrode layer, the second electrode layer comprises multiple horizontal electric field generation parts, upper surfaces of the horizontal electric field generation parts are flush with those of the first electrode strips, and in a row or column direction of the matrix, orthogonal projections, on the base substrate, of the first electrode strips alternate with those of the horizontal electric field generation parts. The invention also provides a display device and a manufacturing method of array substrate. By using the array substrate provided by the invention to display, storage capacitance and driving voltage of each pixel unit are lowered without damaging the overall transmittance in the pixel regions, and uniformity of transmittance in the display area is improved.