Provided is a method for detecting an audio input interface, comprising: acquiring input signals of every one of audio input interfaces; acquiring energy values of said input signals of every one of said audio input interfaces, and acquiring an interface identification of the audio input interface of which the energy value is the maximum among the energy values; adding said interface identification acquired herein into an identification sequence in an order by acquiring time; and identifying the audio input interface, of which the interface identification is saved most often in said identification sequence, as a valid audio input interface. Also provided is a system for detecting an audio input interface. The present disclosure is capable of effectively identifying the audio input interface connected to the microphone that the user is using, so that the user does not need to switch manually.