A novel real-time cargo condition management system and a related method of operation utilizes a unique data synchronization between a remote real-time vehicle on-board diagnostics (OBD) monitoring architecture and a radio frequency identification (RFID) tag attached to a cargo container to track real-time conditions of the cargo container. For example, the cargo container's currently-experienced acceleration and interior temperature can be accurately tracked by a remote monitoring station through a unique data synchronization among a vehicle OBD unit in a truck, an RFID reader installed in the cargo holding area of the truck that periodically scans the RFID tag attached to the cargo container, and an OBD data transceiver unit that wirelessly transmits vehicle OBD data correlated to the RFID tag attached to the cargo container. Similarly, the cargo container's location can be accurately tracked by synchronizing the RFID tag information to the OBD data transceiver unit.