The present disclosure provides a display substrate and a display device, which display substrate including a base substrate a plurality rows of subpixel units formed on the base substrate. Each of the subpixel units is of a parallelogram shape including first sides parallel to the row direction and second sides inclined with respect to vertical direction, wherein the vertical direction is perpendicular to the row direction, second sides of subpixel units in the same row have consistent incline direction, and second sides of subpixel units in adjacent two rows have opposite incline directions. With the display substrate provided in the present disclosure, a display mode of two-pixels-two-domains pixel structure may be implemented by designing subpixel units in the display substrate as parallelograms and making subpixel units in adjacent two rows to have opposite incline directions, which allows to effectively increase viewing angle and reduce color shift while the display substrate is used for virtual display.