A display substrate, display device and driving method thereof. While providing an increased resolution, the display device also has an increased aperture ratio. The display substrate comprises a plurality of pixel units (10) arranged in a matrix. The R sub-pixel, G sub-pixel, and B sub-pixel in each of the plurality of pixel units (10) are connected to a first data line (31), wherein the first data line (31) is configured to provide an R signal to the R sub-pixel in a first time duration (t1), and a B signal to the B sub-pixel in a second time duration (t2). In each of the plurality of pixel units (10), the G sub-pixel is connected to a second data line (32) configured to provide a G signal to the G sub-pixel in the first time duration (t1) and second time duration (t2).