The present disclosure provides a display panel separation pillar and a method for manufacturing the same, a display panel and a display device. The display panel separation pillar includes a first material pattern and a second material pattern on the first material pattern. The first material pattern includes an upper surface and a lower surface opposite to each other, and a first separation lateral side and a second separation lateral side which are opposite to each other and between the upper surface and the lower surface. The second material pattern includes an upper surface and a lower surface opposite to each other. The upper surface of the first material pattern directly contacts with the lower surface of the second material pattern. Projections of the first separation lateral side and the second separation lateral side of the first material pattern onto a plane of the lower surface of the second material pattern are between edges of the lower surface of the second material pattern.