Invention Grant
- Patent Title: Mutant lactobacillus beta-glucuronidase enzymes with enhanced enzymatic activity
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Application No.: US15076183Application Date: 2016-03-21
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Publication No.: US09909111B2Publication Date: 2018-03-06
- Inventor: Jia Yang , Gary Horvath , Pongkwan Sitasuwan , Margarita Marinova , Qian Wang , Lim Andrew Lee
- Applicant: INTEGRATED MICRO-CHROMATOGRAPHY SYSTEMS
- Applicant Address: US SC Irmo
- Assignee: INTEGRATED MICRO-CHROMATOGRAPHY SYSTEMS
- Current Assignee: INTEGRATED MICRO-CHROMATOGRAPHY SYSTEMS
- Current Assignee Address: US SC Irmo
- Agency: Nelson Mullins Riley & Scarborough LLP
- Agent Jill Gorny Sloper, Esq.
- Main IPC: C12N9/24
- IPC: C12N9/24 ; C12P17/10 ; C12Q1/40
Abstract:
Mutated Lactobacillus brevis strain 269Y β-glucuronidase enzymes with enhanced enzymatic activity at low pH (e.g., below pH 6.8), as well as enhanced thermostability as compared to wild type enzyme are provided. The enzymes of the invention advantageously allow for accurate analysis of bodily samples for the presence of drugs at low pH and in 30 minutes or less, as compared to the several hours needed using prior enzyme preparations. Methods of using the mutated enzymes for hydrolysis of glucuronide substrates, including opiates and benzodiazepines, are also provided.
Public/Granted literature
- US20170267985A1 MUTANT LACTOBACILLUS BETA-GLUCURONIDASE ENZYMES WITH ENHANCED ENZYMATIC ACTIVITY Public/Granted day:2017-09-21
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