Invention Grant
- Patent Title: Reduction of lipase activity in product formulations
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Application No.: US15105925Application Date: 2014-12-18
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Publication No.: US09932591B2Publication Date: 2018-04-03
- Inventor: Kelvin Lee , Abraham Lenhoff , Kristin Valente , Nick Levy , Yatin Gokarn
- Applicant: Kelvin Lee , Abraham Lenhoff , Kristin Valente , Nick Levy , Yatin Gokarn
- Applicant Address: US DE Newark
- Assignee: University of Delaware
- Current Assignee: University of Delaware
- Current Assignee Address: US DE Newark
- Agency: RatnerPrestia
- International Application: PCT/US2014/071234 WO 20141218
- International Announcement: WO2015/095568 WO 20150625
- Main IPC: C12N15/11
- IPC: C12N15/11 ; C12N15/113 ; C07K16/00 ; C12P21/02 ; C12N9/20 ; A61K38/17 ; A61K38/39 ; A61K38/42 ; A61K38/44 ; A61K38/45 ; A61K38/48 ; A61K38/52 ; A61K47/26 ; C12P21/00
Abstract:
The invention relates a method for producing a stable recombinant protein, comprising growing a non-naturally occurring host cell in a culture medium to produce a recombinant protein, and making a composition comprising the recombinant protein and a polysorbate. The production of endogenous lipoprotein lipase by the host cell is reduced. The endogenous lipoprotein lipase is present in the composition in a small amount, and is capable of degrading the polysorbate. The invention also relates to the relevant host cells and compositions, and preparation thereof.
Public/Granted literature
- US20160312226A1 REDUCTION OF LIPASE ACTIVITY IN PRODUCT FORMULATIONS Public/Granted day:2016-10-27
Information query
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