Invention Grant
- Patent Title: Genotyping by polymerase binding
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Application No.: US15701373Application Date: 2017-09-11
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Publication No.: US09932631B1Publication Date: 2018-04-03
- Inventor: Corey M. Dambacher , Michael Van Nguyen
- Applicant: Omniome, Inc.
- Applicant Address: US CA San Diego
- Assignee: OMNIOME, INC.
- Current Assignee: OMNIOME, INC.
- Current Assignee Address: US CA San Diego
- Agency: Kilpatrick Townsend & Stockton LLP
- Main IPC: C12Q1/68
- IPC: C12Q1/68

Abstract:
A method for identifying target alleles, that includes steps of (a) forming a plurality of stabilized ternary complexes at a plurality of features on an array, wherein the stabilized ternary complexes each has a polymerase, a template nucleic acid having a target allele of a locus, a primer hybridized to the locus, and a next correct nucleotide having a cognate in the locus, wherein either (i) the primer is an allele-specific primer having a 3′ nucleotide that is a cognate nucleotide for the target allele, or (ii) the primer is a locus-specific primer and the next correct nucleotide hybridizes to the target allele; and (b) detecting stabilized ternary complexes at the features, thereby identifying the target alleles.
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