- Patent Title: Method for determining the spatiotemporal distribution of activity of a proteolytic enzyme in a heterogeneous system (variations), a device for realizing same and a method for diagnosing the defects in the hemostatic system on the basis of a change in the spatiotemporal distribution of activity of a proteolytic enzyme in a heterogeneous system
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Application No.: US14234909Application Date: 2012-07-16
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Publication No.: US09938563B2Publication Date: 2018-04-10
- Inventor: Fazoil Inoyatovich Ataullakhanov , Nataljya Mikhajlovna Dashkevich , Mikhail Vladimirovich Ovanesov , Vasilii Ivanovich Sarbash , Mikhail Aleksandrovich Panteleev , Sergey Sergeevich Karamzin , Andrey Yurjevich Kondratovich
- Applicant: Fazoil Inoyatovich Ataullakhanov , Nataljya Mikhajlovna Dashkevich , Mikhail Vladimirovich Ovanesov , Vasilii Ivanovich Sarbash , Mikhail Aleksandrovich Panteleev , Sergey Sergeevich Karamzin , Andrey Yurjevich Kondratovich
- Applicant Address: RU Moscow
- Assignee: OBSCHESTVO S OGRANICHENNOY OTVETSTVENNOSTYU GEMATOLOGICHESKAYA KORPORATSIYA
- Current Assignee: OBSCHESTVO S OGRANICHENNOY OTVETSTVENNOSTYU GEMATOLOGICHESKAYA KORPORATSIYA
- Current Assignee Address: RU Moscow
- Agency: Volpe and Koenig, P.C.
- Priority: RU2011131293 20110726
- International Application: PCT/RU2012/000570 WO 20120716
- International Announcement: WO2013/015717 WO 20130131
- Main IPC: C12Q1/56
- IPC: C12Q1/56 ; C12Q1/37 ; G01N21/64
Abstract:
The invention relates to the field of biotechnology. The method for determining the spatial and temporal distribution of the activity of a proteolytic enzyme in an in vitro heterogeneous system, such as blood or blood plasma, involves the introduction of a luminescent, fluorogenic or chromogenic substrate into a sample with the subsequent release of a detectable tag as the proteolytic enzyme cleaves the substrate, and the recording of the optical characteristics of the sample, which makes it possible to assess the spatial and temporal distribution of the activity of the enzyme. The device for the implementation of the above method comprises an in vitro system, a means for illuminating the sample, a recording means and a control means. A method for diagnosing homeostatic imbalances according to a change in the spatial and temporal distribution of the activity of a proteolytic enzyme in a blood sample is also proposed.
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