Disclosed are a blood sugar detecting method and a cartridge using same. A cartridge according to the present invention comprises: a blood receiving unit for receiving blood injected therein; a separation unit for separating the received blood into glucose and hemoglobin; a first measuring unit for measuring the concentration of the separated glucose; a second measuring unit for measuring the concentration of hemoglobin A1c (HbA1c) in the hemoglobin; and a third measuring unit connected to a second channel, for measuring the total concentration of hemoglobin. According to the present invention, both glucose providing short-term information on blood sugar and hemoglobin A1c providing long-term information on blood sugar can be detected by a single cartridge, thus increasing the efficiency of diabetes management.