A method for forming an immersion agent film between the sample holder and the objective of a microscope, comprising an automatic immersion module for supplying immersion agent from an immersion agent reservoir to a target position on the sample holder or on the positioning stage, the automatic immersion module having a spraying device with a nozzle connected to a pump for the purpose of generating an immersion agent jet. A dosed immersion agent jet is sprayed via the automatic immersion module onto a target position on the sample holder or a position on the positioning stage, at a defined speed.