Provided are a secondary battery separator having good heat durability, high adhesion property with the electrode active material layer formed on the current collector, and good anti-blocking property, and a secondary battery having such a secondary battery separator. The secondary battery separator of the present invention includes an organic separator layer, a heat-durable layer formed adjacent to at least one surface of the organic separator layer, and an adhesive layer formed on the heat-durable layer, wherein the heat-durable layer contains non-conductive particles and a binder, and the adhesive layer contains a particulate polymer having a glass transition temperature (Tg) of 10 to 100° C.