A tissue retrieval system can contain and withdraw excised tissue specimens from within a body cavity. The tissue retrieval system can include a tissue retrieval bag, a foldable actuator, and an introducer. The foldable actuator can be foldable between an insertion configuration for placement through an access device without the aid of a grasper or applicator, and a deployed configuration for supporting the tissue retrieval bag in an open state. The actuator can have a foldable handle to facilitate withdrawal from the body cavity. After insertion of the tissue specimens into the retrieval bag, the retrieval bag is then cinched closed to prevent spillage of its contents and to prevent contamination of the body cavity and body cavity wall during withdrawal of the retrieval bag from within the body cavity.