The present invention relates to an anti-reflective coating composition, to an anti-reflective film using same, and to a method for preparing the anti-reflective film, wherein the anti-reflective coating composition is capable of forming a coating layer that has a low refractive index and adjusting the surface energy. More particularly, the present invention relates to an anti-reflective film of which the reflectance is minimized and the surface energy is adjusted, by forming a coating layer using an anti-reflective coating composition that contains, as a binder, a siloxane compound which is synthesized by reacting organosilane that has a fluroalky group with alkoxysilane at a certain weight ratio, and also relates to a method for preparing the anti-reflective film. The anti-reflective film using the anti-reflective coating composition has excellent anti-reflective performance, and is thus expected to be applicable to various display devices such as a touch film.