Invention Grant
- Patent Title: Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use
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Application No.: US14160282Application Date: 2014-01-21
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Publication No.: US09951373B2Publication Date: 2018-04-24
- Inventor: Brock Binkowski , Lance P. Encell , Mary Hall , Matthew B. Robers , Michael R. Slater , Keith V. Wood , Monika G. Wood
- Applicant: Promega Corporation
- Applicant Address: US WI Madison
- Assignee: Promega Corporation
- Current Assignee: Promega Corporation
- Current Assignee Address: US WI Madison
- Agency: Casimir Jones SC
- Agent David Staple
- Main IPC: C12N9/02
- IPC: C12N9/02 ; C12N1/21 ; C12N15/00 ; C12Q1/66 ; C07D487/04 ; C12N15/52
Abstract:
An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
Public/Granted literature
- US20140223590A1 OPLOPHORUS-DERIVED LUCIFERASES, NOVEL COELENTERAZINE SUBSTRATES, AND METHODS OF USE Public/Granted day:2014-08-07
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