A puncture device and method are disclosed, the puncture device includes a puncture member having a puncture needle for puncturing a biological tissue, a shaft portion, and a connection portion for connecting the puncture needle and the shaft portion to each other. The puncture needle includes a needle main body for puncturing the biological tissue, and an extension needle provided for movement relative to the needle main body along a longitudinal direction of the needle main body and configured to puncture the biological tissue. The puncture device includes, as extension means for moving the extension needle in a direction toward the distal end of the needle main body with respect to the needle main body to extend the puncture needle, and/or a pusher for pushing the extension needle to move in the direction toward the distal end of the needle main body.