• Patent Title: Enzymatic hydrolysis of acylated steryl glycosides and method for treating biofuel
  • Application No.: US14912127
    Application Date: 2014-08-13
  • Publication No.: US09957453B2
    Publication Date: 2018-05-01
  • Inventor: Linda MungerLaura Nystrom
  • Applicant: ETH Zurich
  • Applicant Address: CH Zurich
  • Assignee: ETH Zurich
  • Current Assignee: ETH Zurich
  • Current Assignee Address: CH Zurich
  • Agency: The Webb Law Firm
  • Priority: EP13004067 20130816
  • International Application: PCT/EP2014/067336 WO 20140813
  • International Announcement: WO2015/022367 WO 20150219
  • Main IPC: C12P33/00
  • IPC: C12P33/00 C10L1/04 C11B3/00 C12P7/64
Enzymatic hydrolysis of acylated steryl glycosides and method for treating biofuel
Abstract:
The present invention relates to the use of at least one glycosidase of the class EC 3.2.1.21, EC 3.2.1.4, EC 3.2.1.7, and/or EC 3.2.1.80 for hydrolyzing at least one glycosiding bond in at least one substrate selected from the group of acylated steryl glycosides of the general formula (I): R1—CO—O—[CmHnOx]y—O—R2 wherein R1 is a saturated or non-saturated C1-C30 alkyl, R2 is a sterol moiety, m=5-7, n=9-14 and x=5-7, y=1-5. The present invention relates further to a method of for hydrolysing steryl gylcosides using said enzymes in biofuel, oil and/or fat.
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