Invention Grant
- Patent Title: Full COLD-PCR enrichment with reference blocking sequence
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Application No.: US14107291Application Date: 2013-12-16
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Publication No.: US09957556B2Publication Date: 2018-05-01
- Inventor: Gerassimos Makrigiorgos
- Applicant: Dana-Farber Cancer Institute, Inc.
- Applicant Address: US MA Boston
- Assignee: Dana-Farber Cancer Institute, Inc.
- Current Assignee: Dana-Farber Cancer Institute, Inc.
- Current Assignee Address: US MA Boston
- Agency: Wolf, Greenfield & Sacks, P.C.
- Main IPC: C12Q1/68
- IPC: C12Q1/68
Abstract:
The present invention is directed to methods, compositions and software for enriching low abundance alleles in a sample. It is directed in particular to the use of an excess amount of reference blocking sequence in an amplification reaction mixture in order to improve the enrichment efficiency, and reduce cycle time, of full COLD-PCR.
Public/Granted literature
- US20140106362A1 Full COLD-PCR Enrichment with Reference Blocking Sequence Public/Granted day:2014-04-17
Information query
