The present disclosure provides a touch substrate, its manufacturing method and a display device. The touch substrate includes a base substrate provided with a plurality of via-holes, a first electrode arranged at a first surface of the base substrate and covering the plurality of via-holes, and a plurality of second electrodes arranged at a second surface of the base substrate opposite to the first surface and each located at a position corresponding to a touch region. An elastic conductor is arranged in each via-hole so as to electrically connect the first electrode to at least one of the second electrodes in the case that the base substrate is being pressed. Each second electrode corresponds to at least one elastic conductor. The first electrode is connected to a bias voltage input end, and the second electrodes are each connected to a respective detection signal output end via a respective detection line.